While potatoes are a representative crop in Bolivia, their cultivation requires a significant amount of chemical pesticides. Some seed treatment chemicals used for seed potatoes in Bolivia can be highly toxic. Additionally, farmers face financial constraints that make it difficult to use these pesticides. In this paper, we investigated the potential of applying plant ash to seed potatoes as an eco-friendly alternative for seed potato disinfection. We also examined the effects of seed tuber cutting in combination with the ash treatment. The potato variety used was “Jatun Puka,” a new variety developed in Peru that has been studied at the Bolivian National Institute of Agricultural and Forestry Innovation (INIAF). The experiment was conducted using a Randomized Complete Block Design (RCBD) with four repetitions in Sipe Sipe and Sapanani o f Cochab amb a, and Mairana of S anta Cruz. The r esults s howed that the e ffects of the ash treatment and tuber cutting were significantly influenced by the local environment of each region in Bolivia. The ash treatment was more effective than the others only in the Sapanani region, where the soil pH was low enough to fall within the optimal range for potatoes after the ash treatment. Generally, cutting seed potatoes had a negative effect on yield across all three regions, with the negative impact increasing in proportion to the humidity or precipitation of each area. However, considering the cost of seed potatoes per unit area, cutting them remains an adoptable option under certain circumstances. We hope this research will serve as an important reference for future studies on eco-friendly potato cultivation in Bolivia.